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I mixed 5 ml of detergent with. I added 40 ml of distilled water to the detergent solution, and I stirred the solution. I then broke down the strawberry cells into basic components: I added 10 ml of the extraction buffer solution to a plastic bag.
I removed the leaves and sepals from the strawberry and placed it inside the bag. I removed the air from the bag, and I compressed the strawberry. I crushed the bag against the table and formed a strawberry slurry. I then extracted the strawberry DNA from other large solids in the slurry: I placed a two inch square of cheesecloth over a funnel and secured it with my fingers.
I poured the slurry over the cheesecloth, which separated the large solids; and I collected the liquid and DNA in the test tube. I discarded the strawberry solids that remained in the cheesecloth. Finally, I isolated the DNA from the liquid in the test tube: I filled a quarter of the test tube with strawberry liquid, and I added the same amount of ice cold ethanol.
I placed a long wooden splint into the test tube, and I stirred it without shaking the tube. The DNA became visible in the ethanol layer at the top of the test tubes. The detergent degrades the phospholipid bilayer of the cell and nuclear membranes after the strawberry is crushed.
At this step of the experiment, the detergent dissolves cell and nuclear membranes, and DNA remains in the extraction buffer solution. The strawberry liquid, which contains DNA, is in the container on the bottom. The ethanol is the top layer, and it contains visible strands of DNA.
The DNA is visible because it is not soluble in a nonpolar solvent such as ethanol. The extraction of DNA was facilitated by the presence of eight copies of each chromosome, or an octoploid genome, in the strawberry.
In contrast, humans have a diploid genome, which includes only two copies of each chromosome. I degraded the strawberry cell walls, cell membranes, and nuclear membranes by crushing the berry and mixing it with a buffer of saltwater and dish detergent.
The mashing physically disrupted the cell walls, the detergent dissolved the membrane phospholipid bilayers, and the salt provided optimum osmolarity. The naturally occurring pectinase and cellulase enzymes also promoted cell wall degradation. The large solids were then separated by filtration, and the resulting solution was collected in a test tube.
At that time, the DNA was dissolved in water, which is a polar solvent; and sodium ions bonded with the negatively charged DNA via an ionic bond. After the addition of ethanol, DNA precipitated into the nonpolar layer, which made the long DNA strands easily visible to the naked eye.
If I were to repeat this experiment, I would attempt to extract DNA from other fruits; including other berries, apples, and bananas. I anticipate that it would be more difficult to extract DNA from cells that have fewer copies of chromosomes. Finally, I would vary the amount and timing of salt added to the extraction buffer.Results: Our lab resulted in the extraction of DNA from a pfmlures.com wooden or plastic stick to extract DNA out of the solution by slowly and carefully moving the tip of the stick around the test tube where the strawberry solution and ethanol meet.
DNA Extraction Lab Report: Luis PosadaObjective: (State the objective of this exercise in 1 sentence)The objective of this experiment is the extraction of DNA. To compare the DNA of a type of strawberry that is more disease or frost resistant than other strawberries To study.
The strawberry meets best when all thousands are lab rises to the first-come observation, when the dna extraction limits left, and when the report decision-maker.
I did a science experiment on extracting DNA from green peas, strawberries, and. Lab report and/or discussion. particular experiment because strawberry cell has eight copies of the genome, and it would be easier to extract the DNA since it is a lot per cell whereas most of the organisms would only have one.
DNA Extraction - Strawberry. Strawberries are octoploid, which means they have eight sets of chromosomes. The procedure for extracting DNA from a strawberry is simple, and the results are usually obvious, it is easy to see the white strands of DNA within the pink solution of strawberry juice.
Strawberry dna extraction lab report Nova presents: march translation edited by c.
Science&EnhancedScope&andSequence&–&LifeScience& Virginia’Department’of’Education’©’’’ ’ 4’ Strawberry’DNAExtraction’Lab’. Oct 21, · The purpose of this lab was to extract DNA from a strawberry. Hypothesis: I predict that DNA can be extracted and isolated from strawberry cells by using a DNA extraction buffer and alcohol. Oct 19, · In this experiment, I successfully extracted DNA from a strawberry, and I was able to observe visible strands of DNA. The extraction of DNA was facilitated by the presence of eight copies of each chromosome, or an octoploid genome, in the strawberry.
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